The herb Aconitum carmichaelii is used in Miao medicine to treat rheumatic diseases, but its neurotoxicity poses clinical challenges. This study investigates the use of compatible herbs in Jin-Gu-Lian formula to detoxify Aconitum carmichaelii and the mechanism behind it. Rats were administered Aconitum carmichaelii extract alone or with compatible herbs, showing that the combination ameliorated oxidative damage, prevented abnormality of neurotransmitters and modulated pharmacokinetics. The study suggests that the use of compatible herbs can alleviate the neurotoxicity of Aconitum carmichaelii in traditional medicine.