Researchers established an online method based on CE to screen α-glucosidase inhibitors from traditional Tibetan medicine extracts. The method involved functionalizing the inner wall of the capillary column and immobilizing the α-glucosidase on it. Results showed good repeatability and batch-to-batch reproducibility, with 16 traditional Tibetan medicines screened out with inhibitory activity. Rheum australe showed the strongest inhibitory effect with an inhibitory rate of 83.3 ± 0.4%. The study demonstrated the effectiveness of the method in finding potential enzyme inhibitors.