This study examined the morphology and chemical makeup of two subspecies of Satureja subspicata plants in nine natural populations in the Dinaric Alps. They identified 17 types of n-alkane compounds in the plants and analyzed their relationship to various geographic and bioclimatic factors. The tests showed clear distinctions between the subspecies and correlations with geographical distribution. Exposure to different environments can also affect the n-alkane profile, so care should be taken when taking samples from natural habitats. This study highlights the importance of considering both morphological and chemical traits in distinguishing plant subspecies.