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- PubMed:
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This study analyzed 12 isozyme loci in 14 populations of Cypripedium parviflorum var. pubescens and 8 populations of Cypripedium kentuckiense to determine their genetic similarity and whether C. kentuckiense should be recognized as a distinct species. The two taxa are closely related, but C. kentuckiense has less variation and heterozygosity. It also has a unique allele and multilocus genotype, suggesting it is a distinct species, possibly originating from C. parviflorum. The isozyme data also showed restricted gene flow between the Virginia population of C. kentuckiense and others. This study supports recognizing C. kentuckiense as a distinct species.