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A study investigated Double Stem Cell® (DSC) for its protective effect on Parkinson's disease (PD). MPTP-induced Parkinsonism in mice and genetic model of PD in Drosophila melanogaster were used in the study. MPTP-induced motor deficits were significantly improved by DSC through dose-dependent improvements in motor, cognitive, and motor coordination. Moreover, DSC attenuated neurotoxin-induced oxidative stress and neuroinflammation in mice and Drosophila. The findings suggest that DSC could be a potential alternative treatment option for PD.